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KMID : 1141020100100020033
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Journal of Applied Oriental Medicine
2010 Volume.10 No. 2 p.33 ~ p.40
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Protective Effect of Leaf Extracts from Nelumbo nucifera on DNA Damage Against Oxidative Stress
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Kim Do-Wan
Park Jae-Ho
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Abstract
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Objective: This study was carred out for the protective effect of leaf extracts from Nelumbo nucifera on DNA damage against oxidative stress
Methods: The 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl scavenging assay and Fe2+ chelating assay were performed for antioxidative effect and φX-174 RF I DNA cleavage assay and intracellular DNA damage assay were used for inhibitory effect of intracellular DNA damage.
Results: The DPPH radical and hydroxyl scavenging activities and Fe2+ chelating activity were 90.82, 83.85, and 40.65% at 200 ug/ml. respectively. The similar pattern was found in hot-water extracts with a slightly lower activity. The inhibitory effect of intracelluar DNA damage was significant in both the Nelumbo nucifera extracts of ethylacetate and hot-water. Benzeneacetic acid, 4-Hdibenzoquinoline, Sarpagan-17-ol and Propenoic acid were identified by GC/MS analysis.
Conclusion: These results indicated that the leaf extracts of Nelumbo nucifera can be used as a natural antioxidants, which effectively inhibits the oxidative DNA damage.
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KEYWORD
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Nelumbo nucifera, DPPH, Fe2+ chelating, DNA damage
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